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rabbit polyclonal antibody  (Novus Biologicals)


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    Structured Review

    Novus Biologicals rabbit polyclonal antibody
    Rabbit Polyclonal Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+survivin+antibody/Survivin+Antibody/us12344642-1044-154-157
    Average 94 stars, based on 105 article reviews
    rabbit polyclonal antibody - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    other:

    Article Title: APC/C Cdh1 is required for the termination of chromosomal passenger complex activity upon mitotic exit.
    Article Snippet: Commercial antibodies used were: mouse monoclonal anti-Aurora A, anti-Aurora B, and anti-p27Kip1 antibodies (#610938, #611082 and #610242, respectively; BD Transduction Laboratories); rabbit polyclonal anti-INCENP (#2786; Cell Signaling Technology); rabbit monoclonal anti-FoxM1 p-T600, anti-HA, anti-Aurora A p-T288/Aurora B p-T232/Aurora C p-T198, and anti-vinculin antibodies (#14655, #3724, #2914 and #13901, respectively; Cell Signaling Technology); anti-Cdc23 and anti-INCENP antibodies (#ab72206 and #ab12183; Abcam); rabbit monoclonal anti-Aurora B (#ab45145; Abcam); mouse monoclonal anti-Cdh1/Fzr and anti-Cdc20 antibodies (#K0085-3 and #K0140-3, respectively; MBL); rabbit polyclonal anti-cyclin A antibody (#sc-751; Santa-Cruz Biotechnology); mouse monoclonal anticyclin B1 antibody (#sc-245; Santa-Cruz Biotechnology); anti-Cdc27 and anti-β-actin antibodies (#C7104 and #A5441, respectively; Sigma-Aldrich); mouse monoclonal anti-HA, anti-FLAG, anti-c-Myc, and anti-GFP antibodies (#014-21881, #018-22381, #011-21874 and #012-22541, respectively; Wako); rabbit polyclonal anti-survivin antibody (#NB500201; Novus Biologicals); rabbit polyclonal anti-Cul1 antibody (#71-8700; Thermo Fisher Scientific); and rabbit polyclonal anti-H3 p-S10 antibody (#06-570; Merck Millipore).

    Article Title: APC/C Cdh1 is required for the termination of chromosomal passenger complex activity upon mitotic exit
    Article Snippet: Commercial antibodies used were: mouse monoclonal anti-Aurora A, anti-Aurora B, and anti-p27 Kip1 antibodies (#610938, #611082 and #610242, respectively; BD Transduction Laboratories); rabbit polyclonal anti-INCENP (#2786; Cell Signaling Technology); rabbit monoclonal anti-FoxM1 p-T600, anti-HA, anti-Aurora A p-T288/Aurora B p-T232/Aurora C p-T198, and anti-vinculin antibodies (#14655, #3724, #2914 and #13901, respectively; Cell Signaling Technology); anti-Cdc23 and anti-INCENP antibodies (#ab72206 and #ab12183; Abcam); rabbit monoclonal anti-Aurora B (#ab45145; Abcam); mouse monoclonal anti-Cdh1/Fzr and anti-Cdc20 antibodies (#K0085-3 and #K0140-3, respectively; MBL); rabbit polyclonal anti-cyclin A antibody (#sc-751; Santa-Cruz Biotechnology); mouse monoclonal anti-cyclin B1 antibody (#sc-245; Santa-Cruz Biotechnology); anti-Cdc27 and anti-β-actin antibodies (#C7104 and #A5441, respectively; Sigma-Aldrich); mouse monoclonal anti-HA, anti-FLAG, anti-c-Myc, and anti-GFP antibodies (#014-21881, #018-22381, #011-21874 and #012-22541, respectively; Wako); rabbit polyclonal anti-survivin antibody (#NB500-201; Novus Biologicals); rabbit polyclonal anti-Cul1 antibody (#71-8700; Thermo Fisher Scientific); and rabbit polyclonal anti-H3 p-S10 antibody (#06-570; Merck Millipore).

    Transfection:

    Article Title: Expression and Function of Survivin in Canine Osteosarcoma
    Article Snippet: Transfected cells were washed, fixed with methanol, and air-dried, followed by incubation in rabbit polyclonal anti-survivin antibody (Novus Biologicals) followed by Alexa Fluor 594 goat anti-rabbit IgG (H þ L; Invitrogen).

    Article Title: Involvement of α5 integrin in survivin-mediated osteosarcoma metastasis.
    Article Snippet: The signals were visualized using an enhanced ECL kit from Amersham Pharmacia Biotech. .. Transfected cells were washed, fixed with methanol, and airdried, then incubated with rabbit polyclonal anti-survivin antibody (Novus Biologicals) and secondary fluorescent antibody. ..

    Article Title: Expression and Function of Survivin in Canine Osteosarcoma
    Article Snippet: Osteosarcoma has a highmortality rate and remains in need ofmore effective therapeutic approaches.. Survivin is an inhibitor of apoptosis familymember protein that blocks apoptosis and drives proliferation in human cancer cells where it is commonly elevated.. In this study, we illustrate the superiority of a canine osteosarcomamodel as a translational tool for evaluating survivin-directed therapies, owing to the striking similarities in gross and microscopic appearance, biologic behavior, gene expression, and signaling pathway alterations.

    Incubation:

    Article Title: Expression and Function of Survivin in Canine Osteosarcoma
    Article Snippet: Transfected cells were washed, fixed with methanol, and air-dried, followed by incubation in rabbit polyclonal anti-survivin antibody (Novus Biologicals) followed by Alexa Fluor 594 goat anti-rabbit IgG (H þ L; Invitrogen).

    Article Title: Survivin inhibition via EZN-3042 in canine lymphoma and osteosarcoma.
    Article Snippet: Canine lymphoma (LSA) and osteosarcoma (OS) have high mortality rates and remain in need of more effective therapeutic approaches.. Survivin, an inhibitor of apoptosis (IAP) family member protein that inhibits apoptosis and drives cell proliferation, is commonly elevated in human and canine cancer.. Survivin expression is a negative prognostic factor in dogs with LSA and OS, and canine LSA and OS cell lines express high levels of survivin.

    Article Title: Involvement of α5 integrin in survivin-mediated osteosarcoma metastasis.
    Article Snippet: The signals were visualized using an enhanced ECL kit from Amersham Pharmacia Biotech. .. Transfected cells were washed, fixed with methanol, and airdried, then incubated with rabbit polyclonal anti-survivin antibody (Novus Biologicals) and secondary fluorescent antibody. ..

    Article Title: Expression and Function of Survivin in Canine Osteosarcoma
    Article Snippet: Osteosarcoma has a highmortality rate and remains in need ofmore effective therapeutic approaches.. Survivin is an inhibitor of apoptosis familymember protein that blocks apoptosis and drives proliferation in human cancer cells where it is commonly elevated.. In this study, we illustrate the superiority of a canine osteosarcomamodel as a translational tool for evaluating survivin-directed therapies, owing to the striking similarities in gross and microscopic appearance, biologic behavior, gene expression, and signaling pathway alterations.

    Expressing:

    Article Title: YM155 reverses rapamycin resistance in renal cancer by decreasing survivin.
    Article Snippet: Renal and renal pelvis tumors make up approximately 3 % of all malignancies.. Most of these cases are renal cell carcinomas (RCC), which are genetically and histologically different from renal pelvis tumors. although approximately 60 % of new cases are diagnosed by coincidence, nearly 25 % are metastatic at the time of diagnosis.. Radical or partial nephrectomy is still the standard therapy in localized RCC.

    Immunohistochemical staining:

    Article Title: YM155 reverses rapamycin resistance in renal cancer by decreasing survivin.
    Article Snippet: Renal and renal pelvis tumors make up approximately 3 % of all malignancies.. Most of these cases are renal cell carcinomas (RCC), which are genetically and histologically different from renal pelvis tumors. although approximately 60 % of new cases are diagnosed by coincidence, nearly 25 % are metastatic at the time of diagnosis.. Radical or partial nephrectomy is still the standard therapy in localized RCC.

    Staining:

    Article Title: YM155 reverses rapamycin resistance in renal cancer by decreasing survivin.
    Article Snippet: Renal and renal pelvis tumors make up approximately 3 % of all malignancies.. Most of these cases are renal cell carcinomas (RCC), which are genetically and histologically different from renal pelvis tumors. although approximately 60 % of new cases are diagnosed by coincidence, nearly 25 % are metastatic at the time of diagnosis.. Radical or partial nephrectomy is still the standard therapy in localized RCC.

    Labeling:

    Article Title: YM155 reverses rapamycin resistance in renal cancer by decreasing survivin.
    Article Snippet: Renal and renal pelvis tumors make up approximately 3 % of all malignancies.. Most of these cases are renal cell carcinomas (RCC), which are genetically and histologically different from renal pelvis tumors. although approximately 60 % of new cases are diagnosed by coincidence, nearly 25 % are metastatic at the time of diagnosis.. Radical or partial nephrectomy is still the standard therapy in localized RCC.



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    Figure 4. ESLE decreased the amount of p-Surv <t>(Thr34)</t> in HSC-3 cells in concentration-dependent manner. HSC-3 cells were treated with/without 25 nM YM155 or ESLE in various concentrations. The amount of p-Surv (Thr34) was measured using Western Blot, as outlined in Methods. *Statistical significance (p<0.05) was determined using Tukey’s post-hoc test when compared to the sham group.
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    Histopathologic Images of <t>Survivin</t> immunostaining. A. Tubulovillous Adenoma with Several Survivin-Positive Epithelial Cells (400×). B. Well differentiated adenocarcinoma showing strong expression of survivin in the tumour cells (400×). C and D. A moderately differentiated adenocarcinoma showing noticeable expression of the survivin protein in a significant number of the tumour cells (400×). E. Most of the tumour cells in a poorly differentiated adenocarcinoma display a significant presence of survivin staining (400×). F. Mucinous adenocarcinoma displays a remarkable strong presence of survivin immunostaining (400×).
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    Image Search Results


    Figure 4. ESLE decreased the amount of p-Surv (Thr34) in HSC-3 cells in concentration-dependent manner. HSC-3 cells were treated with/without 25 nM YM155 or ESLE in various concentrations. The amount of p-Surv (Thr34) was measured using Western Blot, as outlined in Methods. *Statistical significance (p<0.05) was determined using Tukey’s post-hoc test when compared to the sham group.

    Journal: The Indonesian Biomedical Journal

    Article Title: Elephantopus scaber Linn. Leaf Extract Sensitizes Doxorubicin in Inducing Apoptosis in HSC-3 Tongue Cancer Cells through Inhibiting Survivin Activity at Thr34

    doi: 10.18585/inabj.v16i4.3096

    Figure Lengend Snippet: Figure 4. ESLE decreased the amount of p-Surv (Thr34) in HSC-3 cells in concentration-dependent manner. HSC-3 cells were treated with/without 25 nM YM155 or ESLE in various concentrations. The amount of p-Surv (Thr34) was measured using Western Blot, as outlined in Methods. *Statistical significance (p<0.05) was determined using Tukey’s post-hoc test when compared to the sham group.

    Article Snippet: After blocking with 5% skim milk in Tris-buffered saline (TBS, 150 nM NaCl and 50 nM Tris-HCl, pH 7.4), the sheets were incubated with rabbit polyclonal anti-β-Actin (Cat. No. 4967; Cell Signaling, Danvers, MA, USA) and rabbit polyclonal anti-phospho-survivin (Thr34) (Cat. No. 8888; Cell Signaling) antibody.

    Techniques: Concentration Assay, Western Blot

    Histopathologic Images of Survivin immunostaining. A. Tubulovillous Adenoma with Several Survivin-Positive Epithelial Cells (400×). B. Well differentiated adenocarcinoma showing strong expression of survivin in the tumour cells (400×). C and D. A moderately differentiated adenocarcinoma showing noticeable expression of the survivin protein in a significant number of the tumour cells (400×). E. Most of the tumour cells in a poorly differentiated adenocarcinoma display a significant presence of survivin staining (400×). F. Mucinous adenocarcinoma displays a remarkable strong presence of survivin immunostaining (400×).

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Survivin expression is associated with lymph node metastasis and short survival in patients with colorectal adenocarcinoma

    doi:

    Figure Lengend Snippet: Histopathologic Images of Survivin immunostaining. A. Tubulovillous Adenoma with Several Survivin-Positive Epithelial Cells (400×). B. Well differentiated adenocarcinoma showing strong expression of survivin in the tumour cells (400×). C and D. A moderately differentiated adenocarcinoma showing noticeable expression of the survivin protein in a significant number of the tumour cells (400×). E. Most of the tumour cells in a poorly differentiated adenocarcinoma display a significant presence of survivin staining (400×). F. Mucinous adenocarcinoma displays a remarkable strong presence of survivin immunostaining (400×).

    Article Snippet: The Anti-Survivin Rabbit Polyclonal antibody (ab24479; Abcam) was used to carry out immunocytochemistry.

    Techniques: Immunostaining, Expressing, Staining

    Categories of  survivin  immunostaining in colorectal cancer and adenomas

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Survivin expression is associated with lymph node metastasis and short survival in patients with colorectal adenocarcinoma

    doi:

    Figure Lengend Snippet: Categories of survivin immunostaining in colorectal cancer and adenomas

    Article Snippet: The Anti-Survivin Rabbit Polyclonal antibody (ab24479; Abcam) was used to carry out immunocytochemistry.

    Techniques: Immunostaining

    Distribution of  survivin  immunoexpression in relation to clinicopathological parameters

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Survivin expression is associated with lymph node metastasis and short survival in patients with colorectal adenocarcinoma

    doi:

    Figure Lengend Snippet: Distribution of survivin immunoexpression in relation to clinicopathological parameters

    Article Snippet: The Anti-Survivin Rabbit Polyclonal antibody (ab24479; Abcam) was used to carry out immunocytochemistry.

    Techniques: Immunostaining

    Overall survival curve (Kaplan Meier) in relation to Survivin immunoexpression in colorectal cancer patients. There is an association between positive Survivin immunostaining and overall survival (Log-rank 4.012, P=0.045).

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Survivin expression is associated with lymph node metastasis and short survival in patients with colorectal adenocarcinoma

    doi:

    Figure Lengend Snippet: Overall survival curve (Kaplan Meier) in relation to Survivin immunoexpression in colorectal cancer patients. There is an association between positive Survivin immunostaining and overall survival (Log-rank 4.012, P=0.045).

    Article Snippet: The Anti-Survivin Rabbit Polyclonal antibody (ab24479; Abcam) was used to carry out immunocytochemistry.

    Techniques: Immunostaining

    Disease-free survival curve (Kaplan Meier) in relation to Survivin immunoexpression in colorectal cancer patients. There is an association between positive Survivin immunostaining and disease-free survival (Log Rank 4.921, P=0.027).

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Survivin expression is associated with lymph node metastasis and short survival in patients with colorectal adenocarcinoma

    doi:

    Figure Lengend Snippet: Disease-free survival curve (Kaplan Meier) in relation to Survivin immunoexpression in colorectal cancer patients. There is an association between positive Survivin immunostaining and disease-free survival (Log Rank 4.921, P=0.027).

    Article Snippet: The Anti-Survivin Rabbit Polyclonal antibody (ab24479; Abcam) was used to carry out immunocytochemistry.

    Techniques: Immunostaining

    Primary antibodies and their conditioning in this study

    Journal: World Journal of Surgical Oncology

    Article Title: Apoptosis-related factors are relevant to progression of pancreatic neuroendocrine tumors

    doi: 10.1186/s12957-023-03267-4

    Figure Lengend Snippet: Primary antibodies and their conditioning in this study

    Article Snippet: Survivin , Rabbit polyclonal (BC008718) , 1/200 , pH 9.0, overnight , Proteintech, Rosemont, IL, USA.

    Techniques: